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mouse monoclonal anti myosin 7a myo7a  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse monoclonal anti myosin 7a myo7a
    Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs <t>(MYO7A</t> +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery
    Mouse Monoclonal Anti Myosin 7a Myo7a, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 97/100, based on 647 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+anti+myosin+7a+myo7a/pmc12409954-175-30-37?v=Developmental+Studies+Hybridoma+Bank
    Average 97 stars, based on 647 article reviews
    mouse monoclonal anti myosin 7a myo7a - by Bioz Stars, 2026-08
    97/100 stars

    Images

    1) Product Images from "Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity"

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    Journal: Journal of Biomedical Science

    doi: 10.1186/s12929-025-01180-4

    Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery
    Figure Legend Snippet: Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery

    Techniques Used: RNA Sequencing

    Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )
    Figure Legend Snippet: Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )

    Techniques Used: Expressing, Confocal Microscopy, Control, Fluorescence

    Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test
    Figure Legend Snippet: Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test

    Techniques Used: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence

    Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test
    Figure Legend Snippet: Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test

    Techniques Used: Expressing, In Vitro, Control, RNAscope, Immunohistochemistry, Fluorescence

    Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test
    Figure Legend Snippet: Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test

    Techniques Used: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence



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    mouse monoclonal anti myosin 7a myo7a  (Developmental Studies Hybridoma Bank)
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    Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs <t>(MYO7A</t> +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery
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    mouse anti myosin 7a myo7a monoclonal antibody  (Developmental Studies Hybridoma Bank)
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    Detailed distribution of Aqp11 mRNA in the organs of Corti on P3 (a–c) and P21 (d–f). In situ hybridization for Aqp11 (a and d), immunohistochemistry for <t>Myo7a</t> (b and e), and merged images (c and f) are shown. Schematic illustrations indicate cells expressing Aqp11 on P3 (g) and P21 (h). On P3 (a–c) and P21 (d–f), Aqp11 mRNA was detected in the OHCs (magenta arrowheads), pillar cells (magenta open arrows), Deiter’s cells (magenta arrows), and Hensen’s cells (magenta open arrowheads), as schematically illustrated (g and h). Abbreviations: i, IHC; o, OHC; p, pillar cell; d, Deiter’s cell; h, Hensen’s cells. Bars indicate 50 μm.
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    Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery

    Journal: Journal of Biomedical Science

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    doi: 10.1186/s12929-025-01180-4

    Figure Lengend Snippet: Effects of subchronic ototoxicity on vestibular function and HC density in the vestibular sensory epithelium. A – D Vestibular function; graphs show the Vestibular Dysfunction Ratings (in mice, A or the Tail-Lift Angle (in rats, B , C , and D ) of the individual animals from which the RNA was extracted for RNA-seq analysis. E Number of hair bundles counted in 1500X magnification SEM images of the central region of the utricles of mice exposed to 0 (CTRL) or 30 mM IDPN. F Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 20 mM of IDPN for 4 weeks. CC crista centre, CP crista periphery, US utricle striola, ULP utricle lateral periphery, UMP utricle medial periphery. G Number of HCs (MYO7A +) in different regions of the vestibular epithelia from rats exposed to 0 or 500 mg/kg/day of streptomycin for 6 weeks. CC crista centre, CP crista periphery, US utricle striola, UP utricle periphery

    Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

    Techniques: RNA Sequencing

    Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )

    Journal: Journal of Biomedical Science

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    doi: 10.1186/s12929-025-01180-4

    Figure Lengend Snippet: Effect of subchronic ototoxicity on the expression of PMCA2, DNER, KCNA10, osteopontin, calretinin, and MYO7A in vestibular sensory epithelium assessed by immunofluorescent labelling and confocal microscopy. A – B Images of whole mount utricles from control and treated rats showing the effects of IDPN ( A ) or streptomycin ( B ) on PCMA2 expression in stereocilia bundles. Images are maximum intensity projections of a stack of 6 planes (2 µm total thickness). C Expression of DNER in the neck region of crista HCs in control and streptomycin rats. Images are maximum intensity projections of a stack of 4 planes (1.2 µm total thickness). D Quantitative analyses of PCMA2 and DNER expression. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 180 hair bundles ( a and b ) or > 400 HCs (c) per animal. ***: p < 0.001, ****: p < 0.0001, Student’s t-test. E Immunolabelling of CASPR1 and KCNA10 in a control crista (upper row) and in a crista of a rat exposed to IDPN for 4 weeks (bottom row). In control tissue, both proteins are located in the calyceal junction area. After IDPN, most afferent calyces show very reduced expression of CASPR1 (arrows), while a few retain a large amount of label (asterisk). The corresponding HCs show a marked depletion of the KCNA10 label in the calyceal junction, although the non-specific label shown by supporting cells with this antibody persisted. F Quantitative analysis of the effect of chronic IDPN ( a ) and streptomycin ( b ) on KCNA10 expression in the calyceal junction area. Bars show mean ± SEM fluorescence intensity values. Each point is from an individual animal, averaging values from > 400 HCs per animal. **: p < 0.01, NS nonsignificant, Student’s t-test. G and H Ostepontin and MYO7A labels in sections of cristae from control rats and IDPN-4wk rats. I Calretinin label in HCII of the periphery of the utricles of control and IDPN-4wk rats. J Quantitative analyses of the effect of chronic IDPN on the expression of osteopontin, MYO7A, and calretinin. Bars show mean ± SEM fluorescence intensity values. Each data point is from an individual animal, averaging values of 30 HCs per animal. ****: p < 0.0001, ns: nonsignificant, Student’s t-test. Scale bars = 10 µm in ( A , B , C , E , and H ); 25 µm in ( G and I )

    Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

    Techniques: Expressing, Confocal Microscopy, Control, Fluorescence

    Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test

    Journal: Journal of Biomedical Science

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    doi: 10.1186/s12929-025-01180-4

    Figure Lengend Snippet: Effect of subchronic IDPN ototoxicity on Vsig10l2 expression in HCs. A Representative images of mRNA expression analysis of Vsig10l2 and of the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control (upper row) and IDPN-4wk (lower row) rats. The asterisks and arrows label one example of HC expressing or not Visg10l2 , respectively. Note the loss of the Vsig10l2 mRNA label after ototoxicity. Scale bar: 10 µm. B Representative images of osteopontin, calretinin, and VSIG10L2 immunoreactivity in the peripheral utricle of control (upper row) and IDPN-4wk (lower row) rats. Note that VSIG10L2 is expressed in HCI (osteopontin + , asterisks) but not HCII (calretinin + , arrows). Note also the decrease in label for all three antibodies. Scale bar: 10 µm. C Quantitative analysis of the number of HCs ( a , c ) and of the RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. D Quantitative analysis of anti-VSIG10L2 immunoreactivity, in the ( a ) striola and b periphery regions of the utricle of control and IDPN-4wk rats. In C and D , the bars show mean ± SEM values and each data point is from an individual animal. **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, ns not significant, Student’s t-test

    Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

    Techniques: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence

    Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test

    Journal: Journal of Biomedical Science

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    doi: 10.1186/s12929-025-01180-4

    Figure Lengend Snippet: Effect of long term low dose streptomycin exposure on Vsig10l2 expression in the rat vestibular crista in vitro. The cristae were placed in culture on postnatal day 1, allowed to mature for 14 days, and exposed to 0.05 µM streptomycin on days 14 to 21. A Representative images of the expression of Vsig10l2 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral area of the control (upper row) and streptomycin (lower row) cristae. Scale bar: 10 µm. B Quantitative analysis of the number of HC in the area ( a , c ) and the puncta of RNA-scope ( b , d ), comparing fluorescence units of the Ppib and Vsig10l2 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista. The bars show mean ± SEM values and each data point is from an individual epithelium. *: p < 0.05, ns: non significant, Student’s t-test

    Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

    Techniques: Expressing, In Vitro, Control, RNAscope, Immunohistochemistry, Fluorescence

    Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test

    Journal: Journal of Biomedical Science

    Article Title: Early downregulation of hair cell (HC)-specific genes in the vestibular sensory epithelium during chronic ototoxicity

    doi: 10.1186/s12929-025-01180-4

    Figure Lengend Snippet: Effect of subchronic ototoxicity on Atf3 expression in HCs. A Representative images of analysis of Atf3 mRNA expression and the control gene Ppib by RNA-scope, together with MYO7A immunohistochemistry in the peripheral crista of control rats (upper row) and IDPN-4wk rats (lower row). Scale bar: 10 µm. B Quantitative analysis of the number of HCs in the area ( a, c ) and of RNA-scope puncta ( b , d ), comparing fluorescence units of Ppib and Atf3 mRNA per HC in the centre ( a , b ) and the periphery ( c , d ) of the crista of control and IDPN-4wk rats. The bars show mean ± SEM values and each data point is from an individual animal. *: p < 0.05, ns: nonsignificant, Student’s t-test

    Article Snippet: For immunohistochemistry of the vestibular sensory epithelia, the following commercial primary antibodies were used at the indicated dilutions: mouse monoclonal anti-contactin-associated protein (CASPR1) (clone K65/35, cat.#MABN69, Millipore, RRID: AB_2083496, 1/400), mouse monoclonal anti-myosin-7A (MYO7A) (cat.# 138-1, supernatant, Developmental Studies Hybridoma Bank, RRID: AB_2282417, 1/100), rabbit anti-potassium voltage-gated channel subfamily A member 10 (KCNA10, Kv1.8)(cat.# APC-157, Alomone Labs, RRID: AB_2341039, 1/200), rabbit anti-MYO7A (cat.# 25–6790, Proteus Biosciences, RRID: AB_10015251, 1/400), rabbit anti-oncomodulin (cat.# OMG4, Swant, RRID:AB_10000346, 1/400), rabbit anti-plasma membrane calcium transporting ATPase 2 (PMCA2, encoded by the Atp2b2 gene) (cat.# PA1-915, Invitrogen, RRID: AB_2243199, 1/400), goat anti-Delta/Notch Like EGF Repeat Containing (DNER) (cat.# AF2254, R&D Systems, RRID:AB_355202, 1/200), goat anti-Osteopontin (encoded by the Spp1 gene) (cat.# AF808, R&D Systems, RRID: AB_2194992, 1/200), and guinea pig anti-calretinin (cat.# 214104, Synaptic Systems, RRID: AB_10635160, 1/500).

    Techniques: Expressing, Control, RNAscope, Immunohistochemistry, Fluorescence

    Detailed distribution of Aqp11 mRNA in the organs of Corti on P3 (a–c) and P21 (d–f). In situ hybridization for Aqp11 (a and d), immunohistochemistry for Myo7a (b and e), and merged images (c and f) are shown. Schematic illustrations indicate cells expressing Aqp11 on P3 (g) and P21 (h). On P3 (a–c) and P21 (d–f), Aqp11 mRNA was detected in the OHCs (magenta arrowheads), pillar cells (magenta open arrows), Deiter’s cells (magenta arrows), and Hensen’s cells (magenta open arrowheads), as schematically illustrated (g and h). Abbreviations: i, IHC; o, OHC; p, pillar cell; d, Deiter’s cell; h, Hensen’s cells. Bars indicate 50 μm.

    Journal: JARO: Journal of the Association for Research in Otolaryngology

    Article Title: Quantitative Analysis of Aquaporin Expression Levels during the Development and Maturation of the Inner Ear

    doi: 10.1007/s10162-016-0607-3

    Figure Lengend Snippet: Detailed distribution of Aqp11 mRNA in the organs of Corti on P3 (a–c) and P21 (d–f). In situ hybridization for Aqp11 (a and d), immunohistochemistry for Myo7a (b and e), and merged images (c and f) are shown. Schematic illustrations indicate cells expressing Aqp11 on P3 (g) and P21 (h). On P3 (a–c) and P21 (d–f), Aqp11 mRNA was detected in the OHCs (magenta arrowheads), pillar cells (magenta open arrows), Deiter’s cells (magenta arrows), and Hensen’s cells (magenta open arrowheads), as schematically illustrated (g and h). Abbreviations: i, IHC; o, OHC; p, pillar cell; d, Deiter’s cell; h, Hensen’s cells. Bars indicate 50 μm.

    Article Snippet: Mouse anti-myosin 7a (Myo7a) monoclonal antibody was purchased from the Developmental Studies Hybridoma Bank (USA), and anti-β-III tubulin (Tuj-1 clone) was purchased from Covance (USA).

    Techniques: In Situ Hybridization, Immunohistochemistry, Expressing

    Comparison of AQP9 localization to phalloidin (Ph) and myosin 7a (Myo7a) localization in the P21 organ of Corti. The localization of AQP9 in the inner and outer pillar cells were co-localized with phalloidin staining of these cells (a and b, arrows and open arrows, respectively). AQP9 was detected in the Deiter’s cups (a and b, filled arrowheads) beneath the OHCs and in the phalangeal processes (a, open arrowheads) between the OHCs. Bars indicate 20 μm.

    Journal: JARO: Journal of the Association for Research in Otolaryngology

    Article Title: Quantitative Analysis of Aquaporin Expression Levels during the Development and Maturation of the Inner Ear

    doi: 10.1007/s10162-016-0607-3

    Figure Lengend Snippet: Comparison of AQP9 localization to phalloidin (Ph) and myosin 7a (Myo7a) localization in the P21 organ of Corti. The localization of AQP9 in the inner and outer pillar cells were co-localized with phalloidin staining of these cells (a and b, arrows and open arrows, respectively). AQP9 was detected in the Deiter’s cups (a and b, filled arrowheads) beneath the OHCs and in the phalangeal processes (a, open arrowheads) between the OHCs. Bars indicate 20 μm.

    Article Snippet: Mouse anti-myosin 7a (Myo7a) monoclonal antibody was purchased from the Developmental Studies Hybridoma Bank (USA), and anti-β-III tubulin (Tuj-1 clone) was purchased from Covance (USA).

    Techniques: Comparison, Staining